Live 3D imaging of mitosis within spheroid

 
 

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SPIM imaging of a live spheroid expressing H2B–HcRed.

Z-stacks of 100 slices at slice spacing of 1µm were recorded every three minutes (10X objective, NA=0.3).

Three-dimensional visualization of an enlarged region showing the progress of two cells through mitosis. This visualization was obtained with the 3D viewer plug-in of Fiji software. Arrows show the two dividing cells.

Visualisation of the isosurfaces corresponding to the 3D reconstruction of the mitotic chromosomes (red) and interphase nuclei (blue) in a region of  the stack shown above.